Volume : 2, Issue : 11, NOV 2016

ARE ANY SPERM CRYOPRESERVATION METHODS SUPERIOR OVER OTHERS? COMPARISON OF MOTILITY, VIABILITY, AND MOTILE SPERM ORGANELLE MORPHOLOGY EXAMINATION (MSOME) OF HUMAN SPERMATOZOA

Nahid Yari, Sahabeh Etebary, Mohammad Ali Khalili, Ali Reza Talebi, Akram Hosseini, Ali Nabi

Abstract

Background: Cryopreservation is an effort to get an optimal number of functional sperms in a clinical setting. Objective: The present study aimed to evaluate the influence of cooling-warming techniques on human sperm motility, viability and motile sperm organelle morphology examination (MSOME). Materials and Methods: Semen samples of 28 normozoospermic men prepared by swim-up were divided into four parts. One served as the control group (Group I) and the other three parts cryopreserved using rapid freezing (Group II), cooling with droplet (Group III), and cooling with open-pulled straws (Group IV). For each group, MSOME scoring was used to assess of the sperm head (Normal head score=2), vacuoles (Lack of vacuole score=3), and basis (Normal base score=1) according to Cassuto scoring (Total =6). Materials and Methods: Semen samples of 28 normozoospermic men prepared by swim-up were divided into four parts. One served as the control group (Group I) and the other three parts cryopreserved using rapid freezing (Group II), cooling with droplet (Group III), and cooling with open-pulled straws (Group IV). For each group, MSOME scoring was used to assess of the sperm head (Normal head score=2), vacuoles (Lack of vacuole score=3), and basis (Normal base score=1) according to Cassuto scoring (Total =6). Results: The slightest amount of decrease in motility was noticed in group IV (P < 0.001). In group II, the minimum level of viability was achieved. In the morphologic assessment, there were no significant differences between group II and group III in class 1 Cassuto scoring (P = 0.162). A significant increase was seen in group IV, though (P < 0.001). Conclusions: Vitrification of human spermatozoa using open-pulled straws method preserved vital parameters of sperm better than other vitrification methodology. The application of MSOME can assist in the selection of the normal quality of spermatozoa in ART programs.

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