Volume : 2, Issue : 5, MAY 2016

DIRECT IMMUNOFLUORESCENCE : REINFORCING ITS ROLE IN SKIN BIOPSY

Dr. Sandhya. V, Dr. Srinivas CS

Abstract

Context : Direct Immunofluorescence plays a vital role in the diagnosis of immune mediated dermatological disorders . The pattern of staining helps in the classification of bullous disorders. Aims and Objectives : The aim of the present study was to analyze the corroboration between  clinical , histopathological and DIF findings in arriving at the final diagnosis. Material and Methods : Histopathology and DIF of 50 cases of clinically suspected immune-mediated diseases were studied and compared. Fluorescein Isothiocyanate –conjugated rabbit antihuman IgG, IgA, IgM, C3 and Fibrinogen was used. Results : DIF was positive in 95.7% ( 44/46 cases )of immune mediated skin lesions , 4 being non immune lesions confirmed by histopathology. DIF was positive in 98.6% of Pemphigus group, 75% of Discoid lupus erythematosus  and 100% each in Bullous Pemphigoid , vasculitis, congenital bullous dermatosis of childhood and lichen planus .Conclusion : With appropriate sampling and good technique, DIF is a useful supplement for an accurate diagnosis of immune –mediated dermatological disorders .A combination of clinical features, histopathology and DIF solves the diagnostic dilemma in complicated cases.

Keywords

immunofluorescence , bullous lesions, skin biopsy.

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