Volume : 2, Issue : 8, AUG 2016

EFFECT OF MYCOTOXINS ON PIGMENT SYNTHESIS IN MUSTARD (BRASSICA JUNCEA L.) SEEDS (VAR.PUSA BOLD).

M.S. AHMAD

Abstract

Mycotoxins are toxic secondary metabolites produced by fungi that usually belong to the genera like Aspergillus, Penicillium and Fusarium. These can be produced on a wide range of agricultural commodities under varied ecological coditions worldwide. Mycotoxin contaminated products cause significant agro-economic and trade problems at every stages of crop cultivation up to marketing.The significance of mycotoxin in International trade is being recognized both by the developed and developing countries by which the export of agricultural commodities is also being affected. Some mycotoxins particularly aflatoxin, citrinin and zearalenone have been analysed as natural contaminats of various crops including mustard from Bihar state at different stages of the crop development, harvesting and storage. In this investigation effects of five different concentrations (viz.,100,250,500,1000 and 2000μg/l) of aflatoxinB ,citrinin and zearalenone were evaluated against synthesis of different 1 pigments (viz., chlorophyll and carotenoids) in the emerging leaves of mustard seeds (var. Pusa bold) during seedling growth.The inhibitory effects of those myctoxins were observed at all concentrations which were directly correlated with the concentration of the treated toxins. Maximum inhibitions in chlorophyll a, chlorophyll b, total chlorophyll and carotenoids were 78.5, 56.7 67.6 and 84.9% in aflatoxinB1 treated seeds whereas 57.8, 38.3, 61.9 and 68.9% and 64.4, 42.7, 59.2 and 76.1% inhibitions were recorded by citrinin and zearalenone, respectively.

Keywords

Mycotoxins, Chlorophyll, Carotenoid and Mustard seeds.

Article : Download PDF

Cite This Article

Article No : 8

Number of Downloads : 1257

References

1. Ahmad, M.S. (1999). Association of fungi with mustard seeds in field and storage with
special reference to mycotoxin production. Ph.D. Thesis, T.M. Bhagalpur University,
Bhagalpur.
2. Ahmad, M.S. (2007). Effect of AflatoxinB1 and Zearalenone on pigment synthesis in
mustard (var. Kranti) seeds. J.Myco. pathol.Res.45(1):129-131.
3. Arnon, D.I. (1949). Copper enzymes in isolated chloroplasts polyphenol oxidase in
Beta vulgaris. Plant Physiol. 24:1-5
4. Davis, B.H. (1969) Carotenoid. in: Chemistry of plant pigments Vol. 2. pp. 38-165.
Gordvin (ed.). Academic Press, London.
5. Kang, M.S. (1970). Pathogenesis in groundnut (Arachishypogaea.) by
AspergillusflavusLink ex Fries. Ph.D. Thesis. Punjab Agricultural University,
Ludhiana.
6. Mehan, V.K. and Chohan J.S. (1974). Virulence of aflatoxigenic and non-toxigenic
isolates of AspergillusflavusLinks ex Fr. on groundnut seedling. Phytopath Medit.
13:108-109.
7. Prasad, G, Sinha, K.K. and Ali, M.M. (1996). Effects of Aflatoxin B on chlorophyll, 1
nucleic acid and protein contents in maize. BiologiaPlanturum. 38(1): 47-50.
8. Schoental, R. and White, A.F. (1965). Aflatoxin and albinism in plants. Nature
205:57-58.
9. Sinha, A.K. (1991). Studies on aflatoxin production in relation to varietal variations in
wheat. Ph. D. Thesis, Bhagalpur University, Bhagalpur.
10. Sinha, K.K. (1993). Investigations on mycotoxin induced physiological responses in
crop plants. Final Technical Report , UGC Project, P.82.
11. Sinha, K.K. and Kumari, P. (1989). Effect of Aflatoxin B on biochemical changes in 1
mung seeds (variety Pusa119 ). Microbios Letters 40:145-149.
12. Sinha, K.K., Kumar Niranjan and Prasad,G.(1992). Use of mustard (Brassica
juceaL.) and gram (Cicerarietinum L.) seedling germination inhibition assay for aflatoxin
B . Mycopathologia. 121:175-178.