Volume : 5, Issue : 8, AUG 2019

MICROPROPAGATION OF GRAPE VARIETY VITIS VINIFERA L-BANGALORE BLUE

DR. PRASANNA PUROHIT

Abstract

Micropropagation has been a well-established methodology for in vitro regeneration of plants. In-vitro micropropagation of Vitis vinifera provides opportunities for increasing plant material for cultivation. Cultures were established and maintained in vitro on MS medium (Murashige and Skoog) supplemented with BAP for shoot induction. Plant regeneration of Vitis vinifera L. was achieved using axillary buds as explants. The MS medium was supplemented with 6.0 - 8.0 µM/L of BAP growth regulators showed variable responses. Formation of roots directly from internodes was seen in 1.5 µM/L of NAA with 0.1µM/L of BAP. Further it was found that the strength of half MS was a better basal medium for induction of roots from nodal segment with auxiliary.

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